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NGSS*High SchoolLife Science

HS-LS4-4: Natural Selection and Adaptation

Also called NGSS HS-LS4-4 · Topic: Natural Selection and Evolution · Life Science

The Official Wording

Construct an explanation based on evidence for how natural selection leads to adaptation of populations.

Clarification statement: Emphasis is on using data to provide evidence for how specific biotic and abiotic differences in ecosystems (such as ranges of seasonal temperature, long-term climate change, acidity, light, geographic barriers, or evolution of other organisms) contribute to a change in gene frequency over time, leading to adaptation of populations.

NGSS Lead States, 2013 · Read it on nextgenscience.org · checked October 15, 2026

What HS-LS4-4 Means in Plain English

Students use data to explain how natural selection makes a population better suited to where it lives. Differences between places, like temperature, acidity, light, toxic soil or a predator, favor certain inherited traits, so the genes behind them grow more common over generations.

Adaptation here means a change in a whole population across generations, not an individual adjusting during its life. It builds on HS-LS4-2 and HS-LS4-3 and leads to HS-LS4-5, where changing environments cause species to spread, split or vanish.

HS-LS4-4 I Can Statements

  • I can explain how natural selection produces adaptation over generations.
  • I can use data to link an environmental factor to trait changes.
  • I can tell individual acclimation apart from population adaptation.

HS-LS4-4 Worked Example

A bead model of one grass population spreading onto the soil of an old copper mine and onto a nearby meadow.

The Question

How does copper-rich mine soil change the share of copper-tolerant plants in a grass population?

Set It Up: Two cups of 50 beads each (25 blue for tolerant seeds, 25 white for non-tolerant), one labeled mine and one meadow, a coin and a data table.

Grass seeds on mine and meadow soil
Mine soilMeadowMine cupMeadow cupCoin
What Students Do
  1. In the mine cup, draw 20 seeds. Blue seeds always survive, and a white seed survives only if two coin tosses both land heads.Draw: 10 blue, 10 white. Survivors: 10 blue, 3 white
  2. Refill the cup to 50 beads in the survivors' proportions to make the next generation.Mine generation 2: 77% tolerant
  3. In the meadow cup, tolerant plants grow slowly and get crowded out: a blue seed survives only on heads, and white seeds always survive.Meadow generation 2: 38% tolerant
  4. Run four generations in each cup and graph percent tolerant.Mine climbs past 95%. Meadow falls below 10%
What They Find

The same starting population became mostly copper-tolerant on mine soil and mostly non-tolerant in the meadow, so the two groups adapted in opposite directions.

Why it works: Copper kills plants without the tolerance trait, while in clean soil tolerance carries a growth cost, so each environment favors different inherited traits and shifts the gene mix over generations.

Common Mistakes with HS-LS4-4

Individuals adapting on purpose

“The grass learned to handle copper”

Try this: Plants already tolerant survived and made seeds. Tolerance spread through reproduction, not because any single plant changed itself.

Confusing acclimation with adaptation

“My dog grew a thick coat, so it adapted to winter”

Try this: Changes in one body during its lifetime are acclimation. Adaptation is an inherited change in a population across many generations.

Thinking a trait is good everywhere

“Tolerant grass is just better grass”

Try this: A trait's value depends on the environment. Copper tolerance helps on mine soil but costs growth in an ordinary meadow.

Where HS-LS4-4 Fits

Practice HS-LS4-4

Graph Paper GeneratorFree ToolStudents graph percent tolerant for both cups across four generations on one set of axes.Line graphTwo data sets
Open

Quick Check

  1. Why did the mine and meadow populations change in opposite directions?
  2. Is a person getting a suntan an adaptation?
  3. Why does adaptation need variation already present in the population?

HS-LS4-4: Other Names

HS-LS4-4NGSS HS-LS4-4Adaptation by natural selectionNatural selection

Official wording from NGSS Lead States. 2013. Next Generation Science Standards: For States, By States. Washington, DC: The National Academies Press. The plain-English lines and everything else on this page are EMStudio’s own explanations, written beside the official wording. They are not part of the standards.

*NGSS is a registered trademark of WestEd. Neither WestEd nor the lead states and partners that developed the Next Generation Science Standards were involved in the production of this product, and do not endorse it.